Subchromoplast Fractionation Protocol for Different Solanaceae Fruit Species

A protocol manuscript (“Subchromoplast Fractionation Protocol for Different Solanaceae Fruit Species”) has been submitted by the coordinating institution of DISCO, RHUL and has been accepted in Bio-protocol. An accompanying video has also been provided with this manuscript.

The article is open access and available via the following link:
http://www.bio-protocol.org/e1861

This protocol allows the separation of plastoglobules and membranes of chromoplasts through a sucrose gradient. The subchromoplast compartments can then be analysed independently. Comparisons between mutant/transgenic genotypes and their backgrounds can be performed accurately with simultaneous processing during the same fractionation run. The procedure was initially developed for ripening tomato fruit but translation to sweet and hot pepper has been shown.

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